120 antibodies abs Search Results


anti ly6g microbeads ultra pure mouse  (Miltenyi Biotec)
97
Miltenyi Biotec anti ly6g microbeads ultra pure mouse
Anti Ly6g Microbeads Ultra Pure Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti kdr antibody  (Miltenyi Biotec)
94
Miltenyi Biotec anti kdr antibody
Anti Kdr Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc conjugated antihumancd31 antibodies  (Miltenyi Biotec)
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Miltenyi Biotec fitc conjugated antihumancd31 antibodies
Fitc Conjugated Antihumancd31 Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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v5 antibodies  (Bethyl)
95
Bethyl v5 antibodies
V5 Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody anti cardiac troponin t  (Miltenyi Biotec)
96
Miltenyi Biotec antibody anti cardiac troponin t
Antibody Anti Cardiac Troponin T, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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affinity purified sheep anti hiv 1 gp120 polyclonal antibody  (Biosynth Carbosynth)
90
Biosynth Carbosynth affinity purified sheep anti hiv 1 gp120 polyclonal antibody
Affinity Purified Sheep Anti Hiv 1 Gp120 Polyclonal Antibody, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti egf receptor egfr  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti egf receptor egfr
FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of <t>EGFR</t> degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.
Anti Egf Receptor Egfr, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd11c magnetic beads  (Miltenyi Biotec)
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Miltenyi Biotec anti cd11c magnetic beads
FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of <t>EGFR</t> degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.
Anti Cd11c Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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guinea pig anti dacsl serum  (Jackson Immuno)
92
Jackson Immuno guinea pig anti dacsl serum
FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of <t>EGFR</t> degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.
Guinea Pig Anti Dacsl Serum, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-afap-110 antibody  (Agenus Inc)
90
Agenus Inc mouse anti-afap-110 antibody
FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of <t>EGFR</t> degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.
Mouse Anti Afap 110 Antibody, supplied by Agenus Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti psa ncam antibody conjugated to apc fluorochrome  (Miltenyi Biotec)
99
Miltenyi Biotec anti psa ncam antibody conjugated to apc fluorochrome
FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of <t>EGFR</t> degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.
Anti Psa Ncam Antibody Conjugated To Apc Fluorochrome, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd8a  (Miltenyi Biotec)
96
Miltenyi Biotec anti cd8a
FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of <t>EGFR</t> degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.
Anti Cd8a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of EGFR degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: NLRP4 negatively regulates autophagic processes through an association with beclin1.

doi: 10.4049/jimmunol.1001654

Figure Lengend Snippet: FIGURE 5. NLRP4 controls the maturation of autophagosome and endosome through the asso- ciation with class C VPS. A–C, HeLa cells sta- bly expressing mRFP-GFP-LC3 were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. A and B, Forty-eight hours following transfection, the cells were fixed and then analyzed under a confocal microscope. A, Representative photomicrographs are shown; scale bar, 20 mm. B, The number of red fluorescent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. C, Forty-eight hours after transfection, the cells were infected with GAS at an MOI of 50 for the in- dicated times, fixed, and analyzed under a confo- cal microscope. The number of yellow fluores- cent dots (.1 mm) in individual cells was counted (n = 10). The graph shows the mean 6 SD. D, A schematic diagram of target step(s) by NLRC4 or NLRP4 in the course of autophagic flux. E, HeLa cells were transfected with control dsRNA or pooled dsRNAs targeting NLRC4 or NLRP4. Forty- eight hours posttransfection, the cells were treated with EGF (200 ng/ml) for the indicated times. The levels of EGFR degradation were examined by immunoblotting analysis. Representative results of at least three independent experiments are shown. The graph shows the mean 6 SD. F, HEK293 cells were transfected with the indicated plasmids. Forty- eight hours after transfection, the cells were sub- jected to immunoprecipitation and immunoblotting analyses. Representative results of at least three in- dependent experiments are shown. *p , 0.05.

Article Snippet: Epidermal growth factor receptor degradation assay Control, NLRC4-knockdown, or NLRP4-knockdown HeLa cells were treated with 200 ng/ml recombinant epidermal growth factor (EGF) (R&D Systems) for the indicated periods and were subjected to immunoblotting analysis with the anti-EGF receptor (EGFR) (1005; Santa Cruz).

Techniques: Expressing, Transfection, Control, Microscopy, Infection, Western Blot, Immunoprecipitation